DNA sequencing with [alpha-33P]-labeled ddNTP terminators a new approach to DNA sequencing with Thermo Sequenase DNA polymerase

DNA sequencing with [alpha-33P]-labeled ddNTP terminators : a new approach to DNA sequencing with Thermo Sequenase DNA polymerase

A new approach to DNA sequencing is described. The method is based on the use of [alpha-33P]-labeled dideoxyribonucleoside triphosphate terminators and Thermo Sequenase DNA polymerase in cycle sequencing. Thermo Sequenase DNA polymerase incorporates ddNTPs as efficiently as dNTPs, allowing the use o...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1991. - 21(1996), 6 vom: 15. Dez., Seite 1132-7
1. Verfasser: Fan, J (VerfasserIn)
Weitere Verfasser: Ranu, R S, Smith, C, Ruan, C, Fuller, C W
Format: Aufsatz
Sprache:English
Veröffentlicht: 1996
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Codon, Terminator DNA Primers DNA, Bacterial Reagent Kits, Diagnostic DNA-Directed DNA Polymerase EC 2.7.7.7