Approaches to detection of distantly related proteins by database searches

The searching of protein databases as a method of identifying newly sequenced genes is commonplace in molecular biology laboratories. However, it is a procedure that is not usually formally taught to students, and method cookbooks discuss it only briefly. This article uses a single family of highly...

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Détails bibliographiques
Publié dans:BioTechniques. - 1991. - 21(1996), 6 vom: 15. Dez., Seite 1118-22, 1124-5
Auteur principal: Cattell, K (Auteur)
Autres auteurs: Koop, B, Olafson, R S, Fellows, M, Bailey, I, Olafson, R W, Upton, C
Format: Article
Langue:English
Publié: 1996
Accès à la collection:BioTechniques
Sujets:Journal Article Research Support, Non-U.S. Gov't DNA, Viral Proteins DNA Glycosylases EC 3.2.2.- N-Glycosyl Hydrolases Uracil-DNA Glycosidase
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520 |a The searching of protein databases as a method of identifying newly sequenced genes is commonplace in molecular biology laboratories. However, it is a procedure that is not usually formally taught to students, and method cookbooks discuss it only briefly. This article uses a single family of highly diverged uracil-DNA glycosylases, which fall into two distinct groups, to highlight some of the difficulties associated with identification of such proteins by database searching 
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