Solid-phase metal chelate assay for quantifying total protein : resistance to chemical interference

Recently, we developed reversible metal chelate stains that are fully compatible with immunoblotting and protein sequencing. Membrane supports are incubated in Ferrozine/ferrous complex followed by ferrocyanide/ferric complex (double-metal chelate [DMC] stain). Proteins are quantified by computerize...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1993. - 21(1996), 5 vom: 21. Nov., Seite 888-92, 894, 896-7
1. Verfasser: Lim, M J (VerfasserIn)
Weitere Verfasser: Patton, W F, Shojaee, N, Shepro, D
Format: Aufsatz
Sprache:English
Veröffentlicht: 1996
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Chelating Agents Coloring Agents Proteins