Detection of cell-surface antigens using antibody-conjugated fluorospheres (ACF) : application for six-color immunofluorescence

Antibody-conjugated fluorospheres (ACF) were used to phenotype murine leukocytes by flow cytometric analysis. Multicolor immunofluoresence (beyond simultaneous 4-color analysis) is limited by the availability of specific antibody-fluorochrome chrome conjugates and even further restricted by the spec...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1991. - 21(1996), 3 vom: 15. Sept., Seite 498-503
1. Verfasser: Beavis, A J (VerfasserIn)
Weitere Verfasser: Pennline, K J
Format: Aufsatz
Sprache:English
Veröffentlicht: 1996
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Comparative Study Journal Article Antibodies, Monoclonal Antigens, Differentiation, T-Lymphocyte Antigens, Ly Antigens, Surface Fluorescent Dyes Histocompatibility Antigens Class II Macrophage-1 Antigen Organometallic Compounds mehr... Organophosphorus Compounds Thy-1 Antigens allophycocyanin Phycocyanin 11016-15-2 Phycoerythrin 11016-17-4 Cascade Blue 1325-87-7 Leukocyte Common Antigens EC 3.1.3.48 Fluorescein-5-isothiocyanate I223NX31W9