Detection of cell-surface antigens using antibody-conjugated fluorospheres (ACF) : application for six-color immunofluorescence

Antibody-conjugated fluorospheres (ACF) were used to phenotype murine leukocytes by flow cytometric analysis. Multicolor immunofluoresence (beyond simultaneous 4-color analysis) is limited by the availability of specific antibody-fluorochrome chrome conjugates and even further restricted by the spec...

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Détails bibliographiques
Publié dans:BioTechniques. - 1991. - 21(1996), 3 vom: 15. Sept., Seite 498-503
Auteur principal: Beavis, A J (Auteur)
Autres auteurs: Pennline, K J
Format: Article
Langue:English
Publié: 1996
Accès à la collection:BioTechniques
Sujets:Comparative Study Journal Article Antibodies, Monoclonal Antigens, Differentiation, T-Lymphocyte Antigens, Ly Antigens, Surface Fluorescent Dyes Histocompatibility Antigens Class II Macrophage-1 Antigen Organometallic Compounds plus... Organophosphorus Compounds Thy-1 Antigens allophycocyanin Phycocyanin 11016-15-2 Phycoerythrin 11016-17-4 Cascade Blue 1325-87-7 Leukocyte Common Antigens EC 3.1.3.48 Fluorescein-5-isothiocyanate I223NX31W9