Simultaneous detection of microorganisms in soil suspension based on PCR amplification of bacterial 16S rRNA fragments

The effect of buffer composition on simultaneous PCR amplification of 16S rRNA gene fragments of five bacterial species was examined using a number of different buffer systems. Tris-based PCR buffers at final concentrations of 10 mM proved unreliable. However, when the final concentration of Tris wa...

Description complète

Détails bibliographiques
Publié dans:BioTechniques. - 1991. - 21(1996), 3 vom: 15. Sept., Seite 463-6, 468, 470-1
Auteur principal: McGregor, D P (Auteur)
Autres auteurs: Forster, S, Steven, J, Adair, J, Leary, S E, Leslie, D L, Harris, W J, Titball, R W
Format: Article
Langue:English
Publié: 1996
Accès à la collection:BioTechniques
Sujets:Comparative Study Technical Report Journal Article Alkanesulfonic Acids Buffers Cyclohexylamines Ethanolamines RNA, Bacterial RNA, Ribosomal, 16S Suspensions plus... Tromethamine 023C2WHX2V Serum Albumin, Bovine 27432CM55Q Ethanolamine 5KV86114PT 3-((1,1-dimethyl-2-hydroxyethyl)amino)-2-hydroxypropanesulfonic acid 68399-79-1 3-(cyclohexylamino)-2-hydroxy-1-propanesulfonic acid 73463-39-5 Ovalbumin 9006-59-1 Myosins EC 3.6.4.1 Carbonic Anhydrases EC 4.2.1.1