Simultaneous detection of microorganisms in soil suspension based on PCR amplification of bacterial 16S rRNA fragments
The effect of buffer composition on simultaneous PCR amplification of 16S rRNA gene fragments of five bacterial species was examined using a number of different buffer systems. Tris-based PCR buffers at final concentrations of 10 mM proved unreliable. However, when the final concentration of Tris wa...
| Veröffentlicht in: | BioTechniques. - 1988. - 21(1996), 3 vom: 15. Sept., Seite 463-6, 468, 470-1 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1996
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Comparative Study Technical Report Alkanesulfonic Acids Buffers Cyclohexylamines Ethanolamines RNA, Bacterial RNA, Ribosomal, 16S Suspensions Tromethamine mehr... |