Use of flow cytometry and RT-PCR for detecting gene expression by single cells

Use of flow cytometry and RT-PCR for detecting gene expression by single cells

We have developed a method for reliably detecting gene expression by individual, phenotypically defined cells. Cells were sorted by flow cytometry into 96-well plates containing a Nonidet P-40 (NP40)-based bysis solution. Reverse transcription (RT) of cellular major histocompatibility complex class...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1991. - 21(1996), 2 vom: 23. Aug., Seite 286-91
1. Verfasser: Gaynor, E M (VerfasserIn)
Weitere Verfasser: Mirsky, M L, Lewin, H A
Format: Aufsatz
Sprache:English
Veröffentlicht: 1996
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. RNA, Messenger RNA, Viral