Detection of protease activity using a fluorescence-enhancement globular substrate
Bovine serum albumin (BSA) highly derivatized with fluorescein isothiocyanate (FITC, isomer I) served as a fluorescent enhancement substrate to measure protease activity. In the native globular BSA structure, the fluorescence of the lysine-conjugated fluorescein moieties was quenched 98%. Proteolyti...
| Veröffentlicht in: | BioTechniques. - 1993. - 20(1996), 2 vom: 29. Feb., Seite 286-91 |
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| Weitere Verfasser: | , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1996
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Alkylating Agents Fluorescent Dyes Iodoacetates Serum Albumin Dithioerythritol 6892-68-8 Endopeptidases EC 3.4.- Chymotrypsin mehr... |