Cycling probe technology with RNase H attached to an oligonucleotide
A streptavidin-RNase H gene fusion was constructed by cloning the Thermus thermophilus RNase H coding sequence in the streptavidin expression vector pTSA18F. The gene was expressed in Escherichia coli, and the resulting fusion protein was purified to apparent homogeneity. The fusion protein was show...
| Veröffentlicht in: | BioTechniques. - 1991. - 20(1996), 2 vom: 29. Feb., Seite 240-8 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1996
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Bacterial Proteins DNA Primers Recombinant Fusion Proteins Biotin 6SO6U10H04 Streptavidin 9013-20-1 Ribonuclease H EC 3.1.26.4 |