Cytosine methylation : quantitation by automated genomic sequencing and GENESCAN analysis
Bisulfite treatment and PCR amplification of genomic DNA permits the methylation analysis of any cytosine residue in a target sequence. By cloning and sequencing the PCR product, the methylation of individual molecules can be determined, whereas direct sequencing of the PCR product can provide an av...
| Veröffentlicht in: | BioTechniques. - 1991. - 21(1996), 1 vom: 17. Juli, Seite 126-33 |
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| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1996
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Comparative Study Journal Article Research Support, Non-U.S. Gov't Coloring Agents DNA Primers Sulfites Cytosine 8J337D1HZY Taq Polymerase EC 2.7.7.- mehr... |