Generating antibodies against secreted proteins using vascular smooth muscle cells transduced with replication-defective retrovirus

Generating antibodies against secreted proteins using vascular smooth muscle cells transduced with replication-defective retrovirus

The traditional method of antibody (Ab) generation requires repeated injections of antigen (Ag). We have developed an alternative method that allows an investigator to generate a polyclonal antiserum with only a cDNA in hand. We cloned a cDNA encoding the coding frame for baboon tissue inhibitor of...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1993. - 20(1996), 4 vom: 01. Apr., Seite 694-701
1. Verfasser: Forough, R (VerfasserIn)
Weitere Verfasser: Hasenstab, D, Koyama, N, Lea, H, Clowes, M, Clowes, A W
Format: Aufsatz
Sprache:English
Veröffentlicht: 1996
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Antibodies Glycoproteins Protease Inhibitors Tissue Inhibitor of Metalloproteinases