Generating antibodies against secreted proteins using vascular smooth muscle cells transduced with replication-defective retrovirus
The traditional method of antibody (Ab) generation requires repeated injections of antigen (Ag). We have developed an alternative method that allows an investigator to generate a polyclonal antiserum with only a cDNA in hand. We cloned a cDNA encoding the coding frame for baboon tissue inhibitor of...
| Publié dans: | BioTechniques. - 1991. - 20(1996), 4 vom: 01. Apr., Seite 694-701 |
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| Auteur principal: | |
| Autres auteurs: | , , , , |
| Format: | Article |
| Langue: | English |
| Publié: |
1996
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Antibodies Glycoproteins Protease Inhibitors Tissue Inhibitor of Metalloproteinases |