Competitive RNA templates for detection and quantitation of growth factors, cytokines, extracellular matrix components and matrix metalloproteinases by RT-PCR

Competitive RNA templates for detection and quantitation of growth factors, cytokines, extracellular matrix components and matrix metalloproteinases by RT-PCR

Detection of low-abundance mRNAs by reverse transcription-polymerase chain reaction (RT-PCR) has become a standard technique to determine gene expression by tissues and cells in culture. The ability to determine relative or absolute copy number of specific mRNAs has been difficult due to inadequate...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1993. - 20(1996), 4 vom: 01. Apr., Seite 670-4
1. Verfasser: Tarnuzzer, R W (VerfasserIn)
Weitere Verfasser: Macauley, S P, Farmerie, W G, Caballero, S, Ghassemifar, M R, Anderson, J T, Robinson, C P, Grant, M B, Humphreys-Beher, M G, Franzen, L, Peck, A B, Schultz, G S
Format: Aufsatz
Sprache:English
Veröffentlicht: 1996
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Technical Report Journal Article Cytokines Extracellular Matrix Proteins Growth Substances RNA, Messenger Metalloendopeptidases EC 3.4.24.-