Strategy for typing human papillomaviruses by RFLP analysis of PCR products and subsequent hybridization with a generic probe

Genital human papillomaviruses (HPV) were detected by PCR using L1 consensus primers MY09 and MY11. To determine the underlying HPV type(s). PCR products were subsequently analyzed employing a combination of restriction fragment length polymorphism (RFLP) and hybridization with a generic oligonucleo...

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Publié dans:BioTechniques. - 1991. - 19(1995), 4 vom: 17. Okt., Seite 632-9
Auteur principal: Meyer, T (Auteur)
Autres auteurs: Arndt, R, Stockfleth, E, Flammann, H T, Wolf, H, Reischl, U
Format: Article
Langue:English
Publié: 1995
Accès à la collection:BioTechniques
Sujets:Journal Article DNA, Viral Oligonucleotide Probes DNA Restriction Enzymes EC 3.1.21.- Deoxyribonuclease BamHI endodeoxyribonuclease DdeI CTGCAG-specific type II deoxyribonucleases EC 3.1.21.4 Deoxyribonucleases, Type II Site-Specific plus... GANTC-specific type II deoxyribonucleases GGCC-specific type II deoxyribonucleases