Bicistronic vector for the creation of stable mammalian cell lines that predisposes all antibiotic-resistant cells to express recombinant protein

We have developed an improved vector for the stable expression of recombinant protein in mammalian cells. In this vector, designated pCIN, both the recombinant cDNA and the neomycin phosphotransferase selection marker are transcribed from a single promoter element. To facilitate translation of the s...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1988. - 20(1996), 1 vom: 05. Jan., Seite 102-4, 106, 108-10
1. Verfasser: Rees, S (VerfasserIn)
Weitere Verfasser: Coote, J, Stables, J, Goodson, S, Harris, S, Lee, M G
Format: Aufsatz
Sprache:English
Veröffentlicht: 1996
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Technical Report DNA Primers DNA, Complementary Receptors, Serotonin Recombinant Proteins Phosphotransferases (Alcohol Group Acceptor) EC 2.7.1.- Kanamycin Kinase EC 2.7.1.95