Oligonucleotide activation of the type IIe restriction enzyme NaeI for digestion of refractory sites
Certain restriction endonucleases previously shown to exhibit DNA site preferences have a two-site DNA cleavage mechanism. These type IIe restriction endonucleases include NaeI, NarI, EcoRII, HpaII and SacII. Because of this two-site mechanism, it is often difficult or impossible to achieve complete...
| Veröffentlicht in: | BioTechniques. - 1993. - 19(1995), 6 vom: 01. Dez., Seite 990-3 |
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| Format: | Aufsatz |
| Sprache: | English |
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1995
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article DNA, Viral Oligonucleotides endodeoxyribonuclease NaeI EC 3.1.21.- Deoxyribonucleases, Type II Site-Specific EC 3.1.21.4 |