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NLM087195283 |
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DE-627 |
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20250130160316.0 |
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tu |
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231222s1995 xx ||||| 00| ||eng c |
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|a pubmed25n0291.xml
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|a (DE-627)NLM087195283
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|a (NLM)8747660
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|a DE-627
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|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Chomczynski, P
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Short technical reports. Modification of the TRI reagent procedure for isolation of RNA from polysaccharide- and proteoglycan-rich sources
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| 264 |
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1 |
|c 1995
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 11.10.1996
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| 500 |
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|a Date Revised 09.01.2024
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| 500 |
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|a published: Print
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| 500 |
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|a Citation Status MEDLINE
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| 520 |
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|a A modification of the TRI Reagent procedure has been elaborated for isolation of RNA from polysaccharide- and proteoglycan-rich material. In the modified procedure, RNA is precipitated from the aqueous phase by the combined action of isopropanol and a high-salt concentration. Under these conditions, RNA is effectively precipitated while contaminating polysaccharides and proteoglycans remain in the soluble form. The modified precipitation does not prolong or increase the complexity of the TRI Reagent procedure. The new procedure was tested by isolation of RNA from polysaccharide- and proteoglycan-rich tissues such as rat liver and aorta
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| 650 |
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4 |
|a Journal Article
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| 650 |
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7 |
|a Indicators and Reagents
|2 NLM
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| 650 |
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7 |
|a Polysaccharides
|2 NLM
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| 650 |
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7 |
|a Proteoglycans
|2 NLM
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| 650 |
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7 |
|a RNA
|2 NLM
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| 650 |
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7 |
|a 63231-63-0
|2 NLM
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| 650 |
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7 |
|a 1-Propanol
|2 NLM
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| 650 |
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7 |
|a 96F264O9SV
|2 NLM
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| 650 |
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7 |
|a RNA-Directed DNA Polymerase
|2 NLM
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| 650 |
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7 |
|a EC 2.7.7.49
|2 NLM
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| 700 |
1 |
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|a Mackey, K
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1993
|g 19(1995), 6 vom: 01. Dez., Seite 942-5
|w (DE-627)NLM012627046
|x 0736-6205
|7 nnns
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| 773 |
1 |
8 |
|g volume:19
|g year:1995
|g number:6
|g day:01
|g month:12
|g pages:942-5
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