Subtractive hybridization strategy using paramagnetic oligo(dT) beads and PCR
Subtractive hybridization has been widely used for the identification of differentially expressed genes. Here we describe a simple, sensitive strategy of subtractive hybridization that involves binding the driver poly(A)+ RNA pool to paramagnetic Dynabeads Oligo (dT)25. After hybridization with targ...
Veröffentlicht in: | BioTechniques. - 1993. - 20(1996), 3 vom: 22. März, Seite 413-9 |
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Format: | Aufsatz |
Sprache: | English |
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1996
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Zugriff auf das übergeordnete Werk: | BioTechniques |
Schlagworte: | Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Technical Report Journal Article DNA, Complementary Oligodeoxyribonucleotides RNA, Messenger oligo (dT) |