Simultaneous detection of TDT-mediated dUTP-biotin nick end-labeling (TUNEL)-positive cells and multiple immunohistochemical markers in single tissue sections

We have modified the terminal deoxynucleotidyl transferase (TDT)-mediated dUTP-biotin nick end-labeling (TUNEL) method to permit the immunogold-silver intensification detection of apoptotic cells in tissue sections. Such sections can subsequently be processed for multi-labeling fluorescence microsco...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1988. - 19(1995), 5 vom: 15. Nov., Seite 800-5
1. Verfasser: Tornusciolo, D R (VerfasserIn)
Weitere Verfasser: Schmidt, R E, Roth, K A
Format: Aufsatz
Sprache:English
Veröffentlicht: 1995
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, U.S. Gov't, P.H.S. Deoxyuracil Nucleotides deoxyuridine triphosphate 1173-82-6 Biotin 6SO6U10H04 DNA Nucleotidylexotransferase EC 2.7.7.31