Secondary structure in the 3' UTR of EGF and the choice of reverse transcriptases affect the detection of message diversity by RT-PCR
The secondary structure in mRNA is essential for many processes, but it can present a technical problem in making full-length cDNA with reverse transcriptases. Furthermore, different reverse transcriptases have differing abilities to transcribe through regions with secondary structure, which can alt...
| Veröffentlicht in: | BioTechniques. - 1993. - 19(1995), 5 vom: 15. Nov., Seite 806-12, 814-5 |
|---|---|
| 1. Verfasser: | |
| Weitere Verfasser: | , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1995
|
| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. RNA, Messenger Recombinant Proteins Epidermal Growth Factor 62229-50-9 RNA, Transfer 9014-25-9 RNA-Directed DNA Polymerase mehr... |