Detection of nucleic acids in the attomole range using polybiotinylated oligonucleotide probes
This article describes the optimization of the hybridization signal obtained with biotinylated oligonucleotides. Optimal number and positions of biotin moieties on a 33-base oligonucleotide probe were determined. The quality of avidin-peroxidase conjugate and the choice of chromogenic substrate infl...
| Veröffentlicht in: | BioTechniques. - 1988. - 19(1995), 2 vom: 15. Aug., Seite 286-90 |
|---|---|
| 1. Verfasser: | |
| Weitere Verfasser: | , , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1995
|
| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't DNA Probes DNA, Bacterial Oligonucleotides Biotin 6SO6U10H04 |