Screening for point mutations by semi-automated DNA sequencing using sequenase and magnetic beads
We have established an improved method for detecting point mutations by semi-automated DNA sequencing of PCR fragments generated from genomic DNA. The method employs magnetic beads to create immobilized single-stranded DNA templates, and the sequencing reaction is performed with Sequenase. This meth...
| Veröffentlicht in: | BioTechniques. - 1993. - 14(1993), 4 vom: 04. Apr., Seite 618-23 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1993
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Apolipoproteins B Receptors, LDL DNA 9007-49-2 Taq Polymerase EC 2.7.7.- bacteriophage T7 induced DNA polymerase DNA-Directed DNA Polymerase |