Analysis of synthetic oligodeoxyribonucleotides by capillary gel electrophoresis and anion-exchange HPLC
Polyacrylamide slab gel electrophoresis has been traditionally used for the analysis of synthetic oligodeoxyribonucleotides. As the demand for oligonucleotides of high purity has increased, a need for additional high resolution analytical techniques has arisen. This report describes the principles a...
| Veröffentlicht in: | BioTechniques. - 1993. - 14(1993), 4 vom: 04. Apr., Seite 598-606 |
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| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1993
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Oligodeoxyribonucleotides oligo (dT) oligodeoxyadenylic acid |
| Zusammenfassung: | Polyacrylamide slab gel electrophoresis has been traditionally used for the analysis of synthetic oligodeoxyribonucleotides. As the demand for oligonucleotides of high purity has increased, a need for additional high resolution analytical techniques has arisen. This report describes the principles and application of capillary gel electrophoresis and high-performance liquid chromatography for the analysis of synthetic DNA varying in base composition and length. From the results of a series of comparative experiments, the relative advantages and disadvantages of each technique are discussed |
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| Beschreibung: | Date Completed 27.05.1993 Date Revised 15.11.2007 published: Print Citation Status MEDLINE |
| ISSN: | 0736-6205 |