Monitoring coupling of peptides to carrier proteins using biotinylated peptide
The amount of synthetic peptide coupled to a carrier protein is a critical parameter in production of antipeptide antibodies. In the method described, biotinylated peptide was included as a tracer to monitor the coupling of peptide to keyhole limpet hemocyanin or BSA. The extent of coupling was asse...
Publié dans: | BioTechniques. - 1991. - 14(1993), 3 vom: 04. März, Seite 464-9 |
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Auteur principal: | |
Format: | Article |
Langue: | English |
Publié: |
1993
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Accès à la collection: | BioTechniques |
Sujets: | Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Carrier Proteins Peptides Biotin 6SO6U10H04 Hemocyanins 9013-72-3 keyhole-limpet hemocyanin |