Two-step "hot" PCR amplification of GC-rich avian c-myc sequences

Two-step "hot" PCR amplification of GC-rich avian c-myc sequences

A new two-step cycle PCR method has been developed for amplification of GC-rich DNA sequences. Using this method, termed "hot PCR," 111 and 179 bp regions (GC contents of 74% and 76%, respectively) of the avian c-myc proto-oncogene were specifically amplified from cloned and genomic DNA. T...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1991. - 14(1993), 3 vom: 04. März, Seite 390-4
1. Verfasser: Schuchard, M (VerfasserIn)
Weitere Verfasser: Sarkar, G, Ruesink, T, Spelsberg, T C
Format: Aufsatz
Sprache:English
Veröffentlicht: 1993
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. DNA Probes DNA 9007-49-2