Biotin-containing protein as a cause of false positive clones in gene probing with streptavidin/biotin
Using a streptavidin/biotin labeling technique, we unintentionally cloned a gene encoding a biotin carboxyl carrier protein, a subunit of biotin-dependent enzymes, from a genomic library of Streptococcus mutans strain UT-041. In colony lifts, the clone reacted positively to the streptavidin-containi...
| Veröffentlicht in: | BioTechniques. - 1993. - 14(1993), 2 vom: 01. Feb., Seite 209-12 |
|---|---|
| 1. Verfasser: | |
| Weitere Verfasser: | , , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1993
|
| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Bacterial Proteins Carrier Proteins DNA Probes Biotin 6SO6U10H04 Streptavidin 9013-20-1 Fatty Acid Synthase, Type II EC 6.- mehr... |