Protecting recognition sequences on DNA by a cleavage-deficient restriction endonuclease

Protecting recognition sequences on DNA by a cleavage-deficient restriction endonuclease

This report describes the use of a biochemical tool that has been developed to aid in the manipulation of DNA. A DNA binding-proficient and cleavage-deficient BamHI mutant protein, E113K, was used in vitro to protect its recognition sequence (5'-GGATCC-3') against the catalytic action of s...

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Veröffentlicht in:BioTechniques. - 1988. - 15(1993), 2 vom: 01. Aug., Seite 310-5
1. Verfasser: Xu, S Y (VerfasserIn)
Weitere Verfasser: Schildkraut, I
Format: Aufsatz
Sprache:English
Veröffentlicht: 1993
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, Non-U.S. Gov't DNA 9007-49-2 Methyltransferases EC 2.1.1.- Dam methyltransferase EC 2.1.1.72 Site-Specific DNA-Methyltransferase (Adenine-Specific) Endodeoxyribonucleases mehr... EC 3.1.- exonuclease Bal 31 EC 3.1.11.- DNA Restriction Enzymes EC 3.1.21.- Deoxyribonuclease BamHI CCCGGG-specific type II deoxyribonucleases EC 3.1.21.4 Deoxyribonucleases, Type II Site-Specific GATC-specific type II deoxyribonucleases