An extended primer set for PCR amplification of murine kappa variable regions
The design and successful usage of an extended primer set for the PCR amplification of murine variable kappa light chain sequences from mouse hybridomas are described. Since some of these primer pairs also amplify the endogenous SP2/0 aberrant light chain sequence, strategies to distinguish the irre...
| Publié dans: | BioTechniques. - 1991. - 15(1993), 2 vom: 01. Aug., Seite 286-92 |
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| Auteur principal: | |
| Autres auteurs: | , , , |
| Format: | Article |
| Langue: | English |
| Publié: |
1993
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| Accès à la collection: | BioTechniques |
| Sujets: | Research Support, U.S. Gov't, P.H.S. Technical Report Journal Article Immunoglobulin Variable Region Immunoglobulin kappa-Chains Oligodeoxyribonucleotides |