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231222s1993 xx ||||| 00| ||eng c |
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|e rakwb
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|a eng
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1 |
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|a Mansfield, E S
|e verfasserin
|4 aut
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|a Alternative labeling techniques for automated fluorescence based analysis of PCR products
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|c 1993
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 20.10.1993
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|a Date Revised 17.11.2004
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|a published: Print
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|a Citation Status MEDLINE
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|a We present here convenient and simple methods that utilize two new fluorescent tags, TOTO-1 and fluorescein-12-dUTP, in conjunction with analysis of PCR products on automated, fluorescence-based electrophoretic instruments. These methods should prove valuable in those applications wherein the effort or expense of covalently attaching a fluorescent tag onto one or both of the PCR primers is not justified. Advantages and disadvantages of the new labeling methods are then reviewed
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|a Technical Report
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|a Journal Article
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7 |
|a Deoxyuracil Nucleotides
|2 NLM
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| 650 |
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|a Fluoresceins
|2 NLM
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| 650 |
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|a Fluorescent Dyes
|2 NLM
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|a Quinolinium Compounds
|2 NLM
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|a Thiazoles
|2 NLM
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| 650 |
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|a fluorescein-11-deoxyuridine triphosphate
|2 NLM
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| 650 |
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|a 134344-32-4
|2 NLM
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| 650 |
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|a 1,1'-(4,4,7,7-tetramethyl-4,7-diazaundecamethylene)bis-4-(3-methyl-2,3-dihydro(benzo-1,3-thiazole)-2-methylidene)quinolinium
|2 NLM
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| 650 |
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7 |
|a 143413-84-7
|2 NLM
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| 700 |
1 |
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|a Kronick, M N
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1993
|g 15(1993), 2 vom: 01. Aug., Seite 274-9
|w (DE-627)NLM012627046
|x 0736-6205
|7 nnns
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| 773 |
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|g volume:15
|g year:1993
|g number:2
|g day:01
|g month:08
|g pages:274-9
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|d 15
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|h 274-9
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