Shadow bands seen when typing polymorphic dinucleotide repeats : some causes and cures

Polymorphic dinucleotide repeats are generally typed by using the PCR to generate products that are resolved on denaturing acrylamide gels. The presence of "shadow bands" on these gels often makes it difficult to score alleles reliably. We have developed procedures that overcome many of th...

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Détails bibliographiques
Publié dans:BioTechniques. - 1991. - 15(1993), 2 vom: 01. Aug., Seite 280-4
Auteur principal: Litt, M (Auteur)
Autres auteurs: Hauge, X, Sharma, V
Format: Article
Langue:English
Publié: 1993
Accès à la collection:BioTechniques
Sujets:Research Support, U.S. Gov't, P.H.S. Technical Report Journal Article Oligonucleotides