In vitro transcription of transfer RNAs with 3'-end modifications
It is difficult to modify the 3'-end of RNA transcribed in vitro from recombinant plasmid or phagemid DNA because of the need to retain a restriction endonuclease recognition site at the downstream end of the template in order to linearize the DNA before transcription. To avoid limitations on t...
| Veröffentlicht in: | BioTechniques. - 1993. - 15(1993), 2 vom: 01. Aug., Seite 264-6 |
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| Format: | Aufsatz |
| Sprache: | English |
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1993
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Research Support, U.S. Gov't, P.H.S. RNA, Transfer 9014-25-9 |