Quantitation of genomic methylation using ligation-mediated PCR
We have developed a new technique for the quantitation of CpG methylation of genomic DNA. This method measures the conversion of a larger amplified DNA fragment to a shorter DNA product correlating with demethylation. The procedure uses pairs of non-isoschizomeric enzymes, one of which is methylatio...
| Veröffentlicht in: | BioTechniques. - 1993. - 15(1993), 4 vom: 01. Okt., Seite 722-9 |
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| Format: | Aufsatz |
| Sprache: | English |
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1993
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Guanine 5Z93L87A1R Cytosine 8J337D1HZY DNA 9007-49-2 Deoxyribonucleases, Type II Site-Specific mehr... |