A general approach for PCR-amplification and sequencing of chloroplast DNA from crude vascular plant and algal tissue

A general approach for PCR-amplification and sequencing of chloroplast DNA from crude vascular plant and algal tissue

A universal and reliable sequencing approach suitable for evolutionary and phylogenetic studies of most plant species has been developed. The initial step in the approach is PCR with primers from conserved regions in the chloroplast genome. Prior to PCR a simple DNA extraction is carried out where l...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1991. - 16(1994), 3 vom: 31. März, Seite 484-94
1. Verfasser: Fangan, B M (VerfasserIn)
Weitere Verfasser: Stedje, B, Stabbetorp, O E, Jensen, E S, Jakobsen, K S
Format: Aufsatz
Sprache:English
Veröffentlicht: 1994
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, Non-U.S. Gov't DNA Primers DNA, Ribosomal RNA, Transfer 9014-25-9