Large-scale supercoiled plasmid preparation by acidic phenol extraction
A novel method for large-scale plasmid preparation is described. Crude extracts are subjected to acidic phenol extraction to remove any contaminants present in the aqueous phase. The supercoiled plasmid DNA, which preferentially remains in the organic phase and inter-phase, is extracted back into th...
| Veröffentlicht in: | BioTechniques. - 1991. - 16(1994), 3 vom: 31. März, Seite 460-3 |
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| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1994
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Technical Report Journal Article DNA, Bacterial DNA, Superhelical Phenols Tromethamine 023C2WHX2V Phenol |