An improved fluor diffusion assay for chloramphenicol acetyltransferase gene expression
We report on a substantial improvement of the widely used fluor diffusion assay for chloramphenicol acetyltransferase (CAT) activity. The stable and inexpensive [3H]NaAcetate along with yeast acetyl CoA synthetase is used to produce [3H]acetyl CoA with high specific radioactivity and high yield. In...
| Veröffentlicht in: | BioTechniques. - 1993. - 16(1994), 2 vom: 26. Feb., Seite 264-5, 268-9 |
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| Format: | Aufsatz |
| Sprache: | English |
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1994
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Comparative Study Research Support, Non-U.S. Gov't Technical Report Journal Article Tritium 10028-17-8 Acetyl Coenzyme A 72-89-9 Chloramphenicol O-Acetyltransferase EC 2.3.1.28 |