Enhancement of baculovirus plaque assay in insect cell monolayers by DEAE-dextran

The presence of DEAE-dextran in the agarose overlay, when titrating wild-type or recombinant baculoviruses by plaque assay, resulted in a higher definition and contrast of viral plaques and increased the plaque number and mean diameter at least by a factor of 2x on day 5 post infection. This increas...

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Détails bibliographiques
Publié dans:BioTechniques. - 1991. - 16(1994), 6 vom: 15. Juni, Seite 1078-81, 1083-5
Auteur principal: Carrascosa, A L (Auteur)
Format: Article
Langue:English
Publié: 1994
Accès à la collection:BioTechniques
Sujets:Research Support, Non-U.S. Gov't Technical Report Journal Article Recombinant Proteins DEAE-Dextran 9015-73-0