In-frame cloning of large synthetic genes using moderate-size oligonucleotides
A method is described that facilitates the cloning of large synthetic genes in plasmid vectors such as pUC and pGEM. This protocol uses unpurified synthetic oligonucleotides of moderate length (ca. 50-90 bp) to construct larger DNA molecules by a reiterative, directional cloning procedure. Open read...
| Veröffentlicht in: | BioTechniques. - 1991. - 16(1994), 4 vom: 01. Apr., Seite 708-15 |
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| 1. Verfasser: | |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1994
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Oligonucleotides PGLa prepropeptide Protein Precursors DNA 9007-49-2 Deoxyribonuclease EcoRI EC 3.1.21.- Deoxyribonuclease HindIII |