Improved fluorescent cycle sequencing protocol allows reading nearly 1000 bases
Recently available thermostable DNA polymerases result in enhanced resolution and accuracy compared to thermal enzymes used previously in fluorescent cycle sequencing. These new enzymes produce less variations in peak intensities, enhance gel resolution and are less sensitive to unspecific terminati...
| Veröffentlicht in: | BioTechniques. - 1988. - 17(1994), 2 vom: 01. Aug., Seite 302, 304, 306 passim |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1994
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Technical Report DNA-Directed DNA Polymerase EC 2.7.7.7 |
| Zusammenfassung: | Recently available thermostable DNA polymerases result in enhanced resolution and accuracy compared to thermal enzymes used previously in fluorescent cycle sequencing. These new enzymes produce less variations in peak intensities, enhance gel resolution and are less sensitive to unspecific termination caused by either DNA structure or impurities in the DNA preparation. Optimization of nucleotide ratios and the usage of high concentrations of detergents in the sequencing reaction result in sequence readings up to 1000 bases and improve overall reliability of the sequencing protocol; this works successfully in about 90% of cases |
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| Beschreibung: | Date Completed 05.01.1995 Date Revised 21.11.2008 published: Print Citation Status MEDLINE |
| ISSN: | 1940-9818 |