Adaptation of the diphenylamine (DPA) assay to a 96-well plate tissue culture format and comparison with the MTT assay

A simple spectrophotometric method for measuring DNA in proliferating cells is described. The method is an adaptation of the widely used diphenylamine (DPA) reaction to examine DNA in cells grown in a 96-well plate. This assay was capable of detecting as little as 10 ng DNA and could be used to meas...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1991. - 17(1994), 1 vom: 01. Juli, Seite 166-71
1. Verfasser: Natarajan, N (VerfasserIn)
Weitere Verfasser: Shambaugh, G E 3rd, Elseth, K M, Haines, G K, Radosevich, J A
Format: Aufsatz
Sprache:English
Veröffentlicht: 1994
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Tetrazolium Salts Thiazoles DNA 9007-49-2 Diphenylamine 9N3CBB0BIQ thiazolyl blue EUY85H477I