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231222s1994 xx ||||| 00| ||eng c |
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|a (NLM)7946297
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|a DE-627
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|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Avaniss-Aghajani, E
|e verfasserin
|4 aut
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| 245 |
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2 |
|a A molecular technique for identification of bacteria using small subunit ribosomal RNA sequences
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| 264 |
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|c 1994
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 07.12.1994
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|a Date Revised 15.11.2006
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|a published: Print
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|a Citation Status MEDLINE
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| 520 |
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|a We have recently developed a novel molecular technique for identification of specific bacterial species within a complex mixture. The technique uses PCR to amplify small subunit ribosomal RNA (SSU rRNA) genes from a mixture of bacteria. One of the PCR primers is labeled with a fluorescent dye to allow detection of the amplified product. The PCR product is then digested with restriction enzymes and a capillary electrophoresis unit equipped with a laser-induced fluorescence detector is employed to analyze the restriction fragments. Only restriction fragments that contain the fluorescent-labeled primer are detected. Generally, the nucleotide sequence of the SSU rRNA genes is unique for each bacterial species. Consequently, the fluorescent-labeled restriction fragments from different bacterial species often have characteristic lengths. Thus, the different fluorescent peaks that appear in a capillary electropherogram correspond to labeled restriction fragments from different bacterial species. This protocol allows us to identify a number of different bacterial species in a complex mixture. Only a minute sample of bacterial DNA and a minimal amount of time (8-10 h) are required for this analysis. The protocol is sensitive, rapid and capable of identifying a broad spectrum of bacterial species
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| 650 |
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|a Journal Article
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| 650 |
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|a Research Support, U.S. Gov't, Non-P.H.S.
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| 650 |
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|a RNA, Ribosomal
|2 NLM
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| 700 |
1 |
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|a Jones, K
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chapman, D
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Brunk, C
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1988
|g 17(1994), 1 vom: 01. Juli, Seite 144-6, 148-9
|w (DE-627)NLM012627046
|x 1940-9818
|7 nnns
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| 773 |
1 |
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|g volume:17
|g year:1994
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|g month:07
|g pages:144-6, 148-9
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