Intra- and extracellular expression of an scFv antibody fragment in E. coli effect of bacterial strains and pathway engineering using GroES/L chaperonins

Intra- and extracellular expression of an scFv antibody fragment in E. coli : effect of bacterial strains and pathway engineering using GroES/L chaperonins

We have studied the influence of bacterial host on the secretion of single-chain Fv antibody fragment (scFv), the production of this antibody fragment as intracellular fusion protein, and the effect of chaperonin coexpression on intracellular antibody expression. Seven bacterial strains were transfo...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1988. - 16(1994), 3 vom: 31. März, Seite 476-7, 480-3
1. Verfasser: Dueñas, M (VerfasserIn)
Weitere Verfasser: Vázquez, J, Ayala, M, Söderlind, E, Ohlin, M, Pérez, L, Borrebaeck, C A, Gavilondo, J V
Format: Aufsatz
Sprache:English
Veröffentlicht: 1994
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Bacterial Proteins Carcinoembryonic Antigen Chaperonin 10 Chaperonin 60 Heat-Shock Proteins Immunoglobulin Fragments Immunoglobulin Variable Region Recombinant Fusion Proteins