Quantitative and sensitive northern blot hybridization using PCR-generated DNA probes labeled with digoxigenin by nick translation

Northern blot hybridization is one of the most convenient methods of detecting an mRNA. Nonradioactive Northern blotting using digoxigenin (DIG) is becoming widely applied because it is rapid and safe. Previous studies have indicated that DIG-labeled RNA probes are suitable for Northern blot hybridi...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1988. - 15(1993), 5 vom: 03. Nov., Seite 880-2
1. Verfasser: Sato, M (VerfasserIn)
Weitere Verfasser: Murao, K, Mizobuchi, M, Takahara, J
Format: Aufsatz
Sprache:English
Veröffentlicht: 1993
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Comparative Study Technical Report DNA Probes Phosphorus Radioisotopes RNA, Messenger Somatostatin 51110-01-1 Pro-Opiomelanocortin 66796-54-1 DNA mehr... 9007-49-2 Digoxigenin NQ1SX9LNAU