Increasing proportions of uracil in DNA substrates increases inhibition of restriction enzyme digests
Techniques that rely upon the incorporation of uracil into DNA are being published with increasing frequency, especially in PCR protocols. We report here the efficiency of 18 type II restriction enzymes to digest PCR amplicons synthesized with varying proportions of TTP to dUTP in the PCR mixture. W...
| Veröffentlicht in: | BioTechniques. - 1988. - 17(1994), 6 vom: 15. Dez., Seite 1086-90 |
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| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1994
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Technical Report Thymine Nucleotides Uracil Nucleotides DNA Restriction Enzymes EC 3.1.21.- |