A vector, pHisGal, allowing bacterial production of proteins fused to a hexahistidine-tagged Gal4 DNA-binding domain
A vector, pHisGal, was constructted that allows isopropyl-beta-D-thiogalactopyranoside (IPTG)-inducible expression of the DNA-binding domain of the yeast transcription factor Gal4 in Escherichia coli. Protein sequences to be tested for transcription regulatory potential in conjunction with the Gal4...
| Veröffentlicht in: | BioTechniques. - 1991. - 17(1994), 4 vom: 03. Okt., Seite 714-6, 718 |
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| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1994
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Technical Report Journal Article DNA-Binding Proteins Fungal Proteins GAL4 protein, S cerevisiae Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins Transcription Factors Histidine 4QD397987E |