Nucleotide sequencing double-stranded plasmids with primers selected from a nonamer library

Nonamer primers, selected from a nonamer library, were tested by sequencing two plasmid subclones containing known insert sequences. These sequences were scanned (nonamer-mapped) against the 2391-member nonamer library to identify all members that share a 100% match at only one site. A total of 59 n...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1988. - 17(1994), 3 vom: 23. Sept., Seite 536-7, 540-4
1. Verfasser: Slightom, J L (VerfasserIn)
Weitere Verfasser: Bock, J H, Siemieniak, D R, Hurst, G D, Beattie, K L
Format: Aufsatz
Sprache:English
Veröffentlicht: 1994
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article DNA Primers Globins 9004-22-2