Large-scale screening of yeast artificial chromosome libraries using PCR
The construction of physical maps of the human genome using sequence-tagged site content mapping requires that thousands of PCR amplifications be performed. On this scale, measures to reduce cost and to increase throughput become serious considerations. We describe relatively simple measures develop...
| Veröffentlicht in: | BioTechniques. - 1988. - 17(1994), 3 vom: 23. Sept., Seite 498-501 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1994
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Technical Report Journal Article |
| Zusammenfassung: | The construction of physical maps of the human genome using sequence-tagged site content mapping requires that thousands of PCR amplifications be performed. On this scale, measures to reduce cost and to increase throughput become serious considerations. We describe relatively simple measures developed in our laboratory that increase the rate at which these reactions can be performed in a cost-effective manner. These measures have been extensively tested in our laboratory and are readily applicable in other laboratories including those performing library screening on a more modest scale |
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| Beschreibung: | Date Completed 10.02.1995 Date Revised 14.11.2007 published: Print Citation Status MEDLINE |
| ISSN: | 0736-6205 |