Improved accuracy in direct automated DNA sequencing of small PCR products by optimizing the template concentration

Improved accuracy in direct automated DNA sequencing of small PCR products by optimizing the template concentration

Data are presented illustrating the optimum concentration range of reverse transcription PCR-generated products under 500 bp for accurate base calling with direct automated DNA sequencing. A 357-bp fragment of the human estrogen receptor, which includes the DNA binding domain of the protein, was use...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1988. - 17(1994), 3 vom: 23. Sept., Seite 478-82
1. Verfasser: Hyder, S M (VerfasserIn)
Weitere Verfasser: Hu, C, Needleman, D S, Sonoda, Y, Wang, X Y, Baker, V V
Format: Aufsatz
Sprache:English
Veröffentlicht: 1994
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Research Support, U.S. Gov't, P.H.S. Technical Report