Nonradioactive, solid-phase DNase I footprints analyzed on an A.L.F. DNA Sequencer
Solid-phase DNase I footprinting provides a powerful tool for analyzing the sequence-specific interactions of DNA binding proteins. Classically this type of assay requires radioactively labeled DNA molecules. Substitution of the isotope by fluorescein labeling of the DNA fragments enables the analys...
| Veröffentlicht in: | BioTechniques. - 1988. - 17(1994), 3 vom: 23. Sept., Seite 474, 476, 478 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1994
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Research Support, Non-U.S. Gov't Technical Report DNA-Binding Proteins Drosophila Proteins Heat Shock Transcription Factors Hsf protein, Drosophila Transcription Factors Deoxyribonuclease I EC 3.1.21.1 |
| Zusammenfassung: | Solid-phase DNase I footprinting provides a powerful tool for analyzing the sequence-specific interactions of DNA binding proteins. Classically this type of assay requires radioactively labeled DNA molecules. Substitution of the isotope by fluorescein labeling of the DNA fragments enables the analysis of footprint patterns on a standard automated laser fluorescent (A.L.F.) DNA Sequencer. The combination of solid-phase footprinting technology and fluorescence-based nonradioactive detection of fragments has unique advantages over established footprinting technologies |
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| Beschreibung: | Date Completed 10.02.1995 Date Revised 16.11.2017 published: Print Citation Status MEDLINE |
| ISSN: | 1940-9818 |