PCR MIMICS : competitive DNA fragments for use as internal standards in quantitative PCR
A rapid and reliable method is described for preparing competitive DNA fragments for quantitative PCR. Synthetic DNAs complementary to previously established PCR primers are ligated together with the primers to both ends of a generic DNA fragment whose length differs from the natural target gene PCR...
| Veröffentlicht in: | BioTechniques. - 1991. - 14(1993), 2 vom: 15. Feb., Seite 244-9 |
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| Format: | Aufsatz |
| Sprache: | English |
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1993
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Interleukin-1 Oncogene Proteins v-erbB RNA, Messenger Retroviridae Proteins, Oncogenic DNA 9007-49-2 Glyceraldehyde-3-Phosphate Dehydrogenases EC 1.2.1.- RNA-Directed DNA Polymerase mehr... |