Primer design for automated DNA sequencing utilizing T7 DNA polymerase and internal labeling with fluorescein-15-dATP
We have identified additional criteria for the walking primer design that improve the success rate of automated fluorescent DNA sequencing using the internal labeling technique and T7 DNA polymerase. These criteria resulted from the evaluation of over 220 sequences generated with walking primers and...
| Veröffentlicht in: | BioTechniques. - 1988. - 18(1995), 4 vom: 16. Apr., Seite 688-97 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1995
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't DNA Primers Deoxyadenine Nucleotides Fluoresceins fluorescein-15-deoxyadenosine triphosphate bacteriophage T7 induced DNA polymerase EC 2.7.7.- RNA-Directed DNA Polymerase EC 2.7.7.49 mehr... |